monoclonal anti mouse type ii collagen cocktail  (Chondrex Inc)

Journal: Prostaglandins, leukotrienes, and essential fatty acids

Article Title: ANTI-INFLAMMATORY PROPERTIES OF PROSTAGLANDIN E2: DELETION OF MICROSOMAL PROSTAGLANDIN E SYNTHASE-1 EXACERBATES NON-IMMUNE INFLAMMATORY ARTHRITIS IN MICE

doi: 10.1016/j.plefa.2013.08.003

Figure Lengend Snippet: Increased severity of inflammatory arthritis in mPGES-1 KO mice. WT and mPGES-1 KO mice were injected with mouse monoclonal anti-type II collagen 5 clone antibody cocktail kit with lipopolysaccharide (LPS) from E. coli 0111:B4 on D0 and boosted with LPS on D3. The arthritis severity was assessed by arthritis score (A) and by the number of inflamed paws (B). The animal body weight (C) was used as a surrogate, whole-body inflammation marker. Data shown are mean ± SE. (*) depicts P ≤ 0.05 as compared to control (Student t-test, n=9). (**) depicts P ≤ 0.05 as compared to control (Mann-Whitney rank sum test, n=9).

Article Snippet: Mice were housed in microisolator cages in a pathogen-free barrier facility, and all experiments were performed under the Institutional Animal Care and Use Committee guidelines as set forth by the University of Kentucky, Lexington KY. Arthritis model CAIA was induced in 8–12 week old, randomly selected male and female mice using mouse monoclonal anti-type II collagen 5 clone antibody cocktail kit with lipopolysaccharide (LPS) from E. coli 0111:B4 (Chondrex) following the manufacturer's instructions.

Techniques: Injection, Marker, MANN-WHITNEY